Rare Event Protocol
The Rare Event Protocol (REP)™ is an unbiased and efficient application of the optical fractionator technique.
The REP is specifically designed to quantify sparse numbers of well-stained objects, e.g., BrDU-positive cells, in stained tissue sections (Mouton, 2011).
The principle and procedures are straightforward. Using the REP one can quantify total number of rare events in a complete reference space in about hour or less. The REP is especially efficient for a total number of rare events numbering about 2000 or less. In contrast to one hour by the REP, using a non-REP approach such counts typically require about five hours or more to obtain the same result.
- Mouton, P.R. Unbiased Stereology: A Concise Guide. The Johns Hopkins University Press, Baltimore, August, 2011.