stereology solutions

Rare Event Protocol

The Rare Event Protocol (REP) is an unbiased and efficient application of the optical fractionator technique.

The REP is specifically designed to quantify sparse numbers of well-stained objects, e.g., BrDU-positive cells, in stained tissue sections (Mouton, 2011).

The principle and procedures are straightforward. Using the  REP one can quantify total number of rare events in a complete reference space in about hour or less. The REP is especially efficient for a total number of rare events numbering about 2000 or less. In contrast to one hour by the REP, using a non-REP approach such counts typically require about five hours or more to obtain the same result.

  1. Mouton, P.R. Unbiased Stereology: A Concise Guide. The Johns Hopkins University Press, Baltimore, August, 2011.