SRC Biosciences has introduced more than a dozen stereology innovations into the industry. Examples include:
Automatic Optical Fractionator™
Stereology innovation for unbiased counts of immunostained cells using the automatic optical fractionator. Fewer errors (more accurate), no user fatigue and 8-10 times higher throughput than conventional manual stereology.
Ref: Mouton, P.R., et al.. Unbiased Estimation Of Cell Number Using The Automatic Optical Fractionator. J Chemical Neuroanatomy, December 2016. Currently in beta testing for 2017 release.
Rare Event Protocol®
Stereology innovation for efficient counts of rare events (cells) in a defined region of interest. Reduces time for unbiased counts of rare events to one hour or less.
Ref: Mouton, P.R. Unbiased Stereology: A Concise Guide, Johns Hopkins University Press, 2011.
Use of isotropic probes (spheres) to quantify Length and Length Density of linear features such as fibers, capillaries, etc. on tissue sections. Since president are isotropic, Length can be quantified on sections cut at any orientation, e.g., coronal, horizontal.
Ref: Mouton, P.R., et al., Stereological Length Estimation Using Spherical Probes, J Microscopy 206: 54-64, 2002.
Unbiased solution for estimating Total Surface Area and Surface Density of cells and other biological objects in sections cut at any orientation.
Ref: Gokhale, A.M., et al., Stereological Estimation Of Surface Area In Thick Transparent Sections Of Arbitrary Orientation Using Virtual Cycloids. J Microscopy 216(Pt1): 25-31, 2004.
Automatic Stereology Thickness™
Automatic determination of sections thickness with particular emphasis on stereology studies.
Ref: Elozory et al., Automatic Section Thickness Determination Using An Absolute Gradient Focus Function. J Microscopy 248(3): 245-59, 2012.